2021년도 제71차 대한통증학회 학술대회 및 연수교육

Background; In the mouse K/BxN model of arthritis, there is an early inflammatory phase and a later post-inflammatory phase. Both phases are associated with a robust tactile allodynia. In previous work, Botulinum toxin was found to be efficacious against on the mechanical allodynia in mono- and poly-neuropathy in the mouse. Here we examined the effects of intraplantar (IPL) Botulinum toxin on mechanical allodynia in the inflammatory and late phase of KBxN arthritic mice.
Methods; Male C57BL/6 mice received intraperitoneal (i.p.) K/BxN serum. After developing mechanical allodynia on late phase (day 28), IPL Botulinum toxin (1 U) and Saline were injected. We examined mechanical allodynia using von Frey hairs at preadministration and at 1, 3, 5, 7, 10, 14, 17, and 21 days after drug administration. We also measured effects of early IPL Botulinum toxin (1 U) treatment at the same time of i.p. K/BxN serum administration compared with vehicle group.
Results: KBxN arthritic mice showed a prominent allodynia. The control group showed no differences for mechanical allodynia. For the Botulinum toxin groups, the paw withdrawal thresholds to mechanical stimuli were significantly reduced versus the pre-administration values and versus the control group (P < 0.05).
Conclusions; Botulinum toxin given IPL yields attenuation of the mechanical allodynia in mice models of KBxN arthritic pain.